Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioreactor Design and Operational System

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prevalent vertebral fractures among urban-dwelling Chinese postmenopausal women: a population-based, randomized-sampling, cross-sectional study.

Archives of osteoporosis·2022

Same author

Ultrasensitive Acetylcholinesterase detection based on a surface-enhanced Raman scattering lever strategy for identifying nerve fibers.

Talanta·2022

Same author

Small but strong: Pivotal roles and potential applications of snoRNAs in hematopoietic malignancies.

Frontiers in oncology·2022

Same author

NRF2 is a critical regulator and therapeutic target of metal implant particle-incurred bone damage.

Biomaterials·2022

Same author

Endoscopic classification and pathological features of primary intestinal lymphangiectasia.

World journal of gastroenterology·2022

Same author

[Reconsideration of the complications of vertebral augmentation surgery for osteoporotic vertebral compression fractures].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2022

Search research articles

Related Experiment Video

Updated: May 19, 2026

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

Engineered bone tissue associated with vascularization utilizing a rotating wall vessel bioreactor.

Masanori Nishi¹, Rena Matsumoto, Jian Dong

¹National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.

Journal of Biomedical Materials Research. Part A

|August 7, 2012

Summary

This study engineered vascularized bone tissue using mesenchymal stem cells (MSCs) and endothelial cells (ECs) in a bioreactor. This novel approach overcomes limitations in bone tissue engineering, paving the way for clinical applications.

More Related Videos

Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models

Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models

Published on: April 3, 2012

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Related Experiment Videos

Last Updated: May 19, 2026

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models

Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models

Published on: April 3, 2012

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Vascularization remains a critical challenge in developing clinically applicable tissue-engineered bone, often leading to cell necrosis.
Existing bone grafting methods have limitations, driving the need for advanced tissue engineering solutions.

Purpose of the Study:

To develop a vascularized bone tissue construct for potential bone grafting applications.
To investigate the co-culture of mesenchymal stem cells (MSCs) and endothelial cells (ECs) for enhanced bone tissue formation.

Main Methods:

Co-culturing bone marrow mesenchymal stem cells (MSCs) with MSC-derived endothelial cells (ECs) within a porous scaffold.
Utilizing a rotating wall vessel (RWV) bioreactor to simulate physiological conditions.
Identifying ECs via immunofluorescence staining (vWF) and flow cytometry (CD31).
Histochemical analysis of generated tissue using various stains (toluidin blue, H&E, osteopontin, osteocalcin, tomato-lectin).

Main Results:

Successfully generated bone tissue exhibiting vascular-like structures.
Demonstrated the presence and integration of endothelial cells within the engineered bone matrix.
Confirmed the osteogenic differentiation of MSCs within the construct.

Conclusions:

Co-culturing MSCs and ECs in an RWV bioreactor promotes the development of vascularized bone tissue.
The dynamic culture environment and cell-cell interactions are crucial for constructing 3D bone tissue with integrated vasculature.
This approach represents a significant advancement towards clinically viable bone tissue engineering.