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A modified simple method for determination of serum α-tocopherol (vitamin E)
Jameel G Jargar1, Shaheenkousar H Hattiwale, Swastika Das
1Department of Physiology, Al-Ameen Medical College, Karnataka, India.
Background:
Vitamin E is one of the important antioxidants linked to regulate various diseases, such as atherosclerosis, hypertension, and male infertility. A relatively simple and economic biochemical modified method has been developed to determine serum α-tocopherol concentration.
Methods:
The current modified method is based on previous Baker and Frank method and the method of Martinek by using 2,2'-bipyridyl, ferric chloride, and xylene. The complex of ferrous ions generated in this reaction with 2,2'-bipyridyl is determined by using a plain enzyme-linked immunosorbent assay microplate (non-antibody coated) at 492 nm.
Results:
The standard curve of this new modified method shows a linearity with correlation r=0.997 (concentration vs. absorbance). The absorbance of this color complex is directly proportional to the α-tocopherol concentration. The sensitivity of this new modified method has been compared and correlated with Baker and Frank method by using 15 human samples (r=0.99, p<0.0001).
Conclusions:
This simple and economic method may be routinely used to analyze α-tocopherol concentration in serum.
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