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Related Experiment Video

Updated: May 19, 2026

Modeling Human Cerebellar Development In Vitro in 2D Structure

Modeling Human Cerebellar Development In Vitro in 2D Structure

Published on: September 16, 2022

Heterochronic protein expression patterns in the developing embryonic chick cerebellum.

E A Gilbert¹, Y H Lim, M K Vickaryous

¹Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road, Guelph, Ontario, Canada.

Anatomical Record (Hoboken, N.J. : 2007)

|August 7, 2012

Summary

The embryonic chick is a valuable model for neural development research. Chick cerebellar development, including Purkinje cell formation and protein expression, occurs earlier than in mice, highlighting heterochrony in neurodevelopment.

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Last Updated: May 19, 2026

Modeling Human Cerebellar Development In Vitro in 2D Structure

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Published on: September 16, 2022

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

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Published on: May 29, 2013

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

Published on: December 14, 2015

Area of Science:

Neuroscience
Developmental Biology
Comparative Anatomy

Background:

The embryonic chick offers advantages for studying neural development due to cost and rapid development.
Chick cerebellar development shares similarities with mice, featuring a comparable embryonic period.

Purpose of the Study:

To investigate in ovo cerebellar development in the chick.
To analyze the expression patterns of Calbindin (CB), Zebrin II (ZII), and Calretinin (CR) during chick cerebellar development.

Main Methods:

In ovo investigation of chick cerebellar development.
Immunohistochemical analysis of Calbindin, Zebrin II, and Calretinin expression during embryonic development.

Main Results:

Asynchronous expression patterns of CB, ZII, and CR were observed during cerebellar development.
Purkinje cells showed distinct expression timelines for CB, ZII, and CR.
Chick cerebellar development events, including Purkinje cell maturation, initiate approximately 5-7 days earlier than in mice.

Conclusions:

The sequence of Purkinje cell development and protein expression is conserved between chicks and mice.
Precocial development in the chick cerebellum suggests a significant role for heterochrony in neurodevelopment.