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Related Experiment Video

Updated: May 19, 2026

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
09:42

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

Published on: June 26, 2019

Estradiol-mediated tumor neo-vascularization.

Shilpi Rajoria1, Robert Suriano, Yushan L Wilson

  • 1Department of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595.

Oncology Letters
|August 7, 2012
PubMed
Summary
This summary is machine-generated.

Estrogen enhances the recruitment of bone marrow-derived endothelial progenitor cells (BM-EPCs) to tumors, promoting new blood vessel growth essential for cancer progression. This finding suggests estrogen

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Isolation and Culture Expansion of Tumor-specific Endothelial Cells
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Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

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Last Updated: May 19, 2026

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
09:42

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

Published on: June 26, 2019

Isolation and Culture Expansion of Tumor-specific Endothelial Cells
10:15

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

Area of Science:

  • Oncology
  • Vascular Biology
  • Endocrinology

Background:

  • Tumor growth and metastasis depend on neo-vascularization, often initiated by bone marrow-derived endothelial progenitor cells (BM-EPCs).
  • BM-EPCs are recruited to sites of inflammation and tumors, but the specific factors driving this recruitment remain under investigation.
  • Estrogen's role in modulating BM-EPC mobilization and its impact on tumor angiogenesis is not fully understood.

Purpose of the Study:

  • To investigate the effect of estrogen on the mobilization of BM-EPCs to tumor sites.
  • To determine if estrogen influences neo-vascularization by affecting BM-EPC recruitment.
  • To explore the potential of targeting estrogen-induced BM-EPC mobilization for cancer therapy.

Main Methods:

  • Utilized Tek/green fluorescent protein (GFP) transgenic mice for fluorescently tagged bone marrow cells.
  • Orthotopically implanted cancer cells in ovariectomized mice, with and without estrogen supplementation.
  • Tracked migration and incorporation of GFP+ BM-EPCs into tumor vasculature using fluorescence microscopy.

Main Results:

  • GFP+ BM-EPCs were successfully tracked and found incorporated within the tumor vasculature.
  • Estrogen supplementation significantly enhanced the mobilization of BM-EPCs to the tumor site.
  • A notable increase in BM-EPCs within the tumor vasculature was observed in estrogen-supplemented mice compared to controls.

Conclusions:

  • Estrogen plays a crucial role in enhancing the mobilization of BM-EPCs to tumor sites.
  • Estrogen may promote tumor neo-vascularization by increasing BM-EPC recruitment.
  • Targeting estrogen-induced BM-EPC mobilization could offer a novel therapeutic strategy for disrupting tumor vasculogenesis and delivering anti-cancer agents.