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DRO1 sensitizes colorectal cancer cells to receptor-mediated apoptosis

Andreas Herbst1, Constanze Bayer, Claudia Wypior

  • 1Second Department of Medicine, University of Munich, Munich, Germany.

Oncology Letters
|August 7, 2012
PubMed

Insights

Down-regulated by oncogenes 1 (DRO1) may suppress tumors by increasing cancer cell death. This study found DRO1 sensitizes colorectal cancer cells to apoptosis by activating the death-inducing signaling complex (DISC).

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Down-regulated by oncogenes 1 (DRO1) is a potential tumor suppressor.
  • DRO1 is frequently downregulated in colorectal cancers.
  • Previous studies suggest DRO1 inhibits tumor growth by sensitizing cells to apoptosis.

Purpose of the Study:

  • To investigate the effect of DRO1 expression on apoptosis in colorectal cancer cell lines.
  • To elucidate the mechanism by which DRO1 influences apoptosis.

Main Methods:

  • Generated DLD1/DRO1 and HCT116/DRO1 cell lines.
  • Cultured colorectal cancer cell lines.
  • Analyzed apoptosis using flow cytometry.

Main Results:

  • DRO1 expression was analyzed in DLD1/DRO1 and HCT116/DRO1 cells.
  • DRO1 sensitizes colorectal cancer cells to receptor-mediated apoptosis.
  • DRO1 promotes the activation of the death-inducing signaling complex (DISC).

Conclusions:

  • DRO1 plays a role in sensitizing colorectal cancer cells to apoptosis.
  • Activation of the DISC is involved in DRO1-mediated apoptosis.
  • Further research into DRO1's mechanism could lead to new cancer therapies.

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