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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...

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Updated: May 19, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

The life sciences mass spectrometry research unit.

Gérard Hopfgartner1, Emmanuel Varesio

  • 1School of Pharmaceutical Sciences University of Geneva, University of Lausanne Life Sciences Mass Spectrometry Quai Ernest-Ansermet, 30 CH-1211 Geneva 4, Switzerland. Gerard.Hopfgartner@unige.ch

Chimia
|August 8, 2012
PubMed
Summary

Life Sciences Mass Spectrometry (LSMS) develops new analytical methods for complex biological samples. Recent work includes drug analysis in hair, drug metabolism studies, and precise protein quantitation.

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Proteomics

Background:

  • The Life Sciences Mass Spectrometry (LSMS) unit develops advanced analytical workflows.
  • Innovative mass spectrometry and software tools are crucial for analyzing biological matrices.

Purpose of the Study:

  • To summarize recent advancements in LSMS analytical techniques.
  • To showcase applications in drug analysis, metabolism, metabolomics, and protein quantitation.

Main Methods:

  • Matrix-assisted laser desorption/ionization (MALDI) for mass spectrometry imaging (MSI).
  • High-resolution mass spectrometry for simultaneous qualitative/quantitative analysis.
  • Selected reaction monitoring (SRM) for absolute protein quantitation.

Main Results:

  • Successful application of MALDI-MSI for detecting drugs of abuse in hair.
  • Enabled simultaneous qualitative and quantitative analysis in drug metabolism and metabolomics.
  • Achieved absolute quantitation of proteins using SRM.

Conclusions:

  • LSMS unit demonstrates versatile mass spectrometry applications.
  • Developed novel workflows enhance analysis of low molecular weight compounds, peptides, and proteins.
  • These advancements support complex biological sample analysis.