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Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
Published on: December 24, 2017
A defensin-like peptide from Phaseolus vulgaris cv. 'King Pole Bean'
Jack H Wong1, David C W Ip, T B Ng
1School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong. jack1993@yahoo.com
Abstract:
A 5447 Da antifungal peptide with an N-terminal sequence highly homologous to plant defensins was purified from Phaseolus vulgaris cv. 'King Pole Bean' by anion-exchange chromatography on Q Sepharose and FPLC-gel filtration on Superdex 75. The isolated peptide inhibited growth of a number of fungal species, including Mycosphaerella arachidicola, Saccharomyces cerevisiae and Candida albicans, with IC(50) values of 3.9, 4.0 and 8.4 μM, respectively. Using the membrane non-permeable DNA-binding dye SYTOX green, it was found that the peptide increased the cell membrane permeability of M. arachidicola, S. cerevisiae and C. albicans.
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