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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: May 19, 2026

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Fluorescent transgenic mice suitable for multi-color aggregation chimera studies.

Masato Ohtsuka1, Hiromi Miura, Channabasavaiah B Gurumurthy

  • 1Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan. masato@is.icc.u-tokai.ac.jp

Cell and Tissue Research
|August 8, 2012
PubMed
Summary

Pronuclear Injection-based Targeted Transgenesis (PITT) enables reproducible fluorescent transgenic mice. These mice are valuable for creating multi-colored chimeras to study cell interactions and lineage tracing in developmental biology.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Stem Cell Biology

Background:

  • Conventional random integration methods for generating transgenic mice often result in unreliable transgene expression.
  • Pronuclear Injection-based Targeted Transgenesis (PITT) offers a novel method for generating transgenic mice with faithful and reproducible transgene expression.
  • Fluorescent transgenic mice are crucial tools for studying cellular interactions and lineage tracing in vivo.

Purpose of the Study:

  • To evaluate the suitability of PITT-generated fluorescent transgenic mice for creating multi-colored aggregation chimeras.
  • To assess cell mixing dynamics and cell distribution patterns in chimeric embryos and adult mice derived from PITT lines.

Main Methods:

  • Generation of fluorescent transgenic mouse lines using the Pronuclear Injection-based Targeted Transgenesis (PITT) method.
  • Aggregation of two- or three-colored 8-cell stage embryos to create chimeric blastocysts and chimeric transgenic mice.
  • Analysis of cell mixing during morula to blastocyst stages and cell distribution in fetal and adult chimeric organs.

Main Results:

  • Cell mixing was observed to be infrequent during the morula to early blastocyst stages.
  • Chimeric fetuses derived from aggregated two-colored embryos displayed uniform cell mixing.
  • Adult chimeric mouse organs exhibited either clonal or polyclonal cell distribution, consistent with previous findings.

Conclusions:

  • PITT-generated fluorescent transgenic mice are suitable for creating multi-colored aggregation chimeras.
  • This improved approach facilitates detailed clonal cell lineage and developmental studies in living systems.
  • The study validates the utility of PITT for generating advanced models for developmental and cellular research.