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Healthcare Associated Infections II: Preventive Measures

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Updated: May 19, 2026

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

Decreasing operating room environmental pathogen contamination through improved cleaning practice.

L Silvia Munoz-Price¹, David J Birnbach, David A Lubarsky

¹Department of Medicine, University of Miami, Miami, Florida, USA. smunozprice@med.miami.edu

Infection Control and Hospital Epidemiology

|August 8, 2012

Summary

Improved operating room cleaning using UV markers and Gram staining feedback enhanced disinfection thoroughness. However, pathogenic organism recovery rates from surfaces did not significantly change overall.

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Last Updated: May 19, 2026

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

Area of Science:

Infection Control and Hospital Epidemiology
Environmental Hygiene in Healthcare Settings

Background:

Operating rooms require stringent disinfection due to potential pathogen transmission.
Environmental contamination poses a risk to patient safety in healthcare settings.

Purpose of the Study:

To assess the impact of feedback interventions on operating room cleaning thoroughness.
To evaluate changes in pathogenic organism recovery from operating room surfaces post-intervention.

Main Methods:

Prospective environmental study utilizing UV markers for cleaning assessment.
Environmental cultures were used to detect pathogenic organisms before and after intervention.
Intervention involved feedback on cleaning using Gram staining and UV marker results.

Main Results:

Cleaning thoroughness, measured by UV marker removal, significantly increased from 47% to 82%.
Overall recovery of pathogenic organisms (Staphylococcus aureus, Enterococcus species) did not change.
A significant decrease in gram-negative bacilli recovery from 10.7% to 2.3% was observed.

Conclusions:

Feedback methods, including UV markers and Gram staining, effectively improved operating room cleaning.
While cleaning improved, the reduction in specific pathogens like gram-negative bacilli warrants further investigation.