Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation. However, because inorganic electron donors...
Methods to Assess Microbial Communities01:19

Methods to Assess Microbial Communities

Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alkamines reveal a hidden layer of steroid and drug metabolism.

bioRxiv : the preprint server for biology·2026
Same author

Serum progesterone and vaginal gel dose in artificial-cycle frozen embryo transfer: association with pregnancy outcome.

Frontiers in endocrinology·2026
Same author

Effects of Sperm DNA Fragmentation on Embryonic Cleavage and Semen Parameters in In Vitro Fertilization Treatment.

Journal of aging research·2026
Same author

Benchmarking Peptide Spectral Library Search.

bioRxiv : the preprint server for biology·2026
Same author

The 2025 Report on the Human Proteome from the HUPO Human Proteome Project.

Journal of proteome research·2026
Same author

Charting the Undiscovered Metabolome with Synthetic Multiplexing.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: May 19, 2026

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

Interkingdom metabolic transformations captured by microbial imaging mass spectrometry.

Wilna J Moree1, Vanessa V Phelan, Cheng-Hsuan Wu

  • 1Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA.

Proceedings of the National Academy of Sciences of the United States of America
|August 8, 2012
PubMed
Summary

Researchers used advanced imaging mass spectrometry to reveal molecular interactions between Pseudomonas aeruginosa and Aspergillus fumigatus. This study uncovers complex metabolic exchanges and biotransformations in polymicrobial infections.

More Related Videos

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
09:59

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)
04:07

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)

Published on: February 23, 2024

Related Experiment Videos

Last Updated: May 19, 2026

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
09:59

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)
04:07

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)

Published on: February 23, 2024

Area of Science:

  • Microbiology
  • Metabolomics
  • Biochemistry

Background:

  • Polymicrobial infections involve complex microbial interactions affecting disease.
  • Cystic fibrosis lung mucus is prone to infections by Pseudomonas aeruginosa and Aspergillus fumigatus.
  • Understanding these interactions requires advanced molecular analysis technologies.

Purpose of the Study:

  • To investigate the interkingdom molecular interactions between P. aeruginosa and A. fumigatus.
  • To identify and visualize secreted metabolites and their exchanges.
  • To elucidate metabolic biotransformations between these two species.

Main Methods:

  • MALDI-TOF and MALDI-FT-ICR imaging mass spectrometry (MALDI-IMS).
  • MS/MS networking for metabolite identification and pathway analysis.
  • Culturing P. aeruginosa and A. fumigatus on agar for analysis.

Main Results:

  • Visualization and identification of secreted metabolites from both species.
  • Revealed complex molecular interplay including metabolite suppression and increased production.
  • Demonstrated biotransformation of P. aeruginosa phenazines by A. fumigatus into novel compounds with altered properties, including enhanced toxicity and induction of fungal siderophores.

Conclusions:

  • MALDI-IMS combined with MS/MS network analysis is effective for studying interkingdom microbial interactions.
  • Significant metabolic exchange and biotransformation occur between P. aeruginosa and A. fumigatus.
  • These findings provide crucial insights into polymicrobial infections, particularly in cystic fibrosis.