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Related Experiment Video

Updated: May 19, 2026

Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Published on: July 29, 2020

Probing regulatory proteins for vascular contraction by deoxyribonucleic Acid microarray.

Jee In Kim¹, In Kyeom Kim

¹Department of Pharmacology, Kyungpook National University School of Medicine, Daegu, Korea.

Korean Circulation Journal

|August 8, 2012

Summary

Heat shock significantly alters vascular smooth muscle contractility by changing gene expression. This study identified novel genes, including heat shock proteins and others like alpha B-crystallin, involved in this heat shock response.

Keywords:

Contraction DNA HSP70 Heat shock Microarray

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Last Updated: May 19, 2026

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Published on: September 14, 2012

Area of Science:

Vascular Biology
Molecular Biology
Genomics

Background:

The heat-shock response is known to influence vascular smooth muscle contractility.
Identifying genes involved in this response is crucial for understanding vascular function.

Purpose of the Study:

To identify novel genes regulated by heat shock in vascular smooth muscle.
To investigate the gene expression profile associated with heat shock in human radial arteries.

Main Methods:

Human radial artery strips were subjected to heat shock (42°C for 45 minutes).
Complementary deoxyribonucleic acid (cDNA) microarray analysis was performed on 5763 human genes.
Gene expression profiles were analyzed using cluster analysis and confirmed with real-time quantitative polymerase chain reaction.

Main Results:

Heat shock significantly increased the expression of heat shock proteins (HSPs) such as Hsp70, Hsp10, and Hsp60.
Non-HSP genes, including alpha B-crystallin (CRYAB), showed increased expression (up 1.92-fold).
Genes related to vascular contraction, such as myosin light chain kinase and tropomyosin 3, were differentially expressed.

Conclusions:

Gene expression profiling following heat shock provides insights into genes modulating vascular contraction.
Alpha B-crystallin and other identified genes are implicated in the augmented vascular contraction observed after heat shock.