Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

Eukaryotic Transcription Inhibitors

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pasteurized Akkermansia muciniphila Muc<sup>T</sup> for weight loss maintenance in people with overweight and obesity: a controlled randomized trial.

Nature medicine·2026

Same author

Genome-wide screen for deficiencies modifying Cyclin G-induced developmental instability in Drosophila melanogaster.

Genetics·2026

Same author

Muscle and Liver Insulin Resistance Are Associated with Distinct Plasma Protein Profiles: Data from the DiOGenes Trial.

The Journal of clinical endocrinology and metabolism·2025

Same author

Quality of platelet concentrates after three-day storage following 265 nm ultraviolet C-light-emitting diode irradiation.

Transfusion medicine (Oxford, England)·2025

Same author

Recent progress in understanding of allergic transfusion reaction.

Current opinion in immunology·2025

Same author

Mapping out the gut microbiota-dependent trimethylamine N-oxide super pathway for systems biology applications.

Frontiers in systems biology·2025

Search research articles

Related Experiment Video

Updated: May 19, 2026

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

MK3 controls Polycomb target gene expression via negative feedback on ERK.

Peggy Prickaerts^1,2, Hanneke Ec Niessen¹, Emmanuèle Mouchel-Vielh²

¹Department of Molecular Genetics, GROW School for Oncology and Developmental Biology, Maastricht University, Universiteitssingel 50, 6229ER, Maastricht, The Netherlands.

Epigenetics & Chromatin

|August 9, 2012

Summary

Mitogen-activated protein kinases (MAPKs) regulate gene transcription via Polycomb repressive complex 1 (PRC1) by controlling its chromatin association. This study reveals MK3

More Related Videos

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

A Method to Study de novo Formation of Chromatin Domains

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

Related Experiment Videos

Last Updated: May 19, 2026

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

A Method to Study de novo Formation of Chromatin Domains

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

Area of Science:

Epigenetics and Gene Regulation
Molecular Biology
Cellular Signaling Pathways

Background:

Gene-environment interactions are mediated by epigenetic mechanisms, involving Polycomb Group proteins in transcriptional memory.
Understanding the dynamic chromatin association of Polycomb repressive complexes (PRCs) during development is limited.
MK3 interacts with Polycomb repressive complex 1 (PRC1), but its functional relevance is poorly understood.

Purpose of the Study:

To investigate how MK3 and its effector kinases (MAPK/ERK, SAPK/P38) regulate PRC1-dependent transcription of the ATF3 gene.
To elucidate the molecular mechanisms controlling PRC1's dynamic chromatin association and dissociation.

Main Methods:

Studied the immediate-early response gene ATF3 as a model for PRC1-dependent transcription.
Utilized cell culture and molecular signaling pathway analysis (ERK, P38, MK3).
Investigated histone modifications (H3S28 phosphorylation, H3K27me3) and protein-chromatin interactions (BMI1, pERK).

Main Results:

Mitogenic signaling via ERK, P38, and MK3 regulates ATF3 expression through PRC1/chromatin dissociation and epigenetic modulation.
ERK signaling drives PRC1 dissociation from chromatin, while P38-dependent H3S28 phosphorylation is insufficient alone.
pERK is a novel signaling-induced binding partner of PRC1; MK3 provides negative feedback on M/SAPKs, supported by Drosophila studies.

Conclusions:

Identified key components of a PRC1-dependent epigenetic signal/response mechanism with modular specificity.
Provided novel insight into Polycomb-mediated epigenetic control of gene transcription.
Established a direct link between PRC1 and cellular responses to microenvironmental changes.