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Related Experiment Video

Updated: May 19, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Lectin-array blotting: profiling protein glycosylation in complex mixtures.

Juan Etxebarria1, Javier Calvo, Manuel Martin-Lomas

  • 1Biofunctional Nanomaterials Unit, San Sebastian, Spain.

ACS Chemical Biology
|August 9, 2012
PubMed
Summary

We developed a novel high-throughput method combining protein electrophoresis and lectin arrays for rapid glycan profiling in biofluids. This unbiased glycoproteomics approach aids biomarker discovery by analyzing protein glycosylation patterns.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Glycomics

Background:

  • Protein glycosylation is crucial for biological functions.
  • Current glycan analysis methods lack high-throughput and unbiased profiling capabilities.
  • Analyzing glycosylation in complex biofluid mixtures is challenging.

Purpose of the Study:

  • To develop a high-throughput method for rapid analysis of protein glycosylation in biofluids.
  • To enable unbiased biomarker discovery through qualitative exploration of protein glycosylation.
  • To combine electrophoretic protein separation with lectin-array-based glycan profiling.

Main Methods:

  • Developed a single-experiment method integrating SDS-PAGE and lectin arrays.
  • Separated fluorescently tagged proteins and transferred them to a lectin-coated slide.

More Related Videos

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
20:23

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

Published on: October 1, 2009

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
07:12

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Published on: September 29, 2023

Related Experiment Videos

Last Updated: May 19, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
20:23

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

Published on: October 1, 2009

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
07:12

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Published on: September 29, 2023

  • Utilized specific carbohydrate-protein interactions for glycan profiling.
  • Verified lectin affinity for N-glycans using a 76-structure glycan array.

    • Main Results:

    • Achieved rapid, high-throughput analysis of protein glycosylation.
    • Generated detailed affinity profiles with 20 different lectins.
    • Obtained structural information on present glycans.
    • Demonstrated the method's utility for unbiased biomarker discovery.

    Conclusions:

    • The developed array-based glycoproteomics method offers a powerful tool for analyzing protein glycosylation in biofluids.
    • This technique facilitates unbiased biomarker discovery for various diseases.
    • It overcomes limitations of current methods by providing comprehensive glycan information.