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Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Updated: May 19, 2026

Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons

Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons

Published on: June 9, 2012

Using ES cells labeled with GFP for analyzing cell behavior during differentiation.

Zhonghua Liu¹, Queenie P Vong, Yixian Zheng

¹Carnegie Institution for Science, Department of Embryology, Baltimore, Maryland, USA.

Current Protocols in Stem Cell Biology

|August 9, 2012

Summary

This summary is machine-generated.

We engineered mouse embryonic stem cells to express GFP for live cell tracking during differentiation. This system aids in studying stem cell behavior and lineage development.

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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

Area of Science:

Stem cell biology
Molecular biology
Developmental biology

Background:

Mouse embryonic stem (ES) cells are crucial for studying stem cell maintenance and differentiation.
Live cell imaging and tracking are essential for understanding dynamic cellular processes.

Purpose of the Study:

To develop a method for generating engineered mouse ES cells for live cell tracing.
To enable the study of cell behavior during differentiation into various lineages.

Main Methods:

Generation of mouse ES cells engineered to stably express Green Fluorescent Protein (GFP).
Creation of cell lines expressing either GFP alone or GFP fused with histone H2B.
Utilizing these engineered cells for live or fixed sample analysis.

Main Results:

Successfully generated mouse ES cells with stable GFP expression.
Developed a system allowing for live or fixed cell tracing.
Demonstrated the utility of the system for observing cell behavior during differentiation.

Conclusions:

Engineered mouse ES cells expressing GFP provide a robust system for live cell tracking.
This method facilitates the investigation of stem cell behavior and lineage commitment.
The developed system is valuable for stem cell research and developmental biology studies.