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A rapid two-step purification of rat liver fetal thymidine kinase
G Baron1, A Madec-Baron, S Sampérez
1Centre Régional de Recherche en Endocrinologie, Faculté de Médecine-Pharmacie, Rennes, France.
Preparative Biochemistry
|January 1, 1990
Abstract:
The fetal isoenzyme of thymidine kinase was purified to apparent homogeneity from cytosols of rat fetuses liver. A two-step purification including anion exchange chromatography and affinity chromatography was developed. The purified enzyme appears as oligomeric with a relative molecular weight of 71 kDa. In denaturing media its molecular weight was 24 kDa, and its pHi 8.3.