Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluation of an Integrated Spectroscopy and Classification Platform for Point-of-Care Core Needle Biopsy Assessment: Performance Characteristics from Ex Vivo Renal Mass Biopsies.

Journal of vascular and interventional radiology : JVIR·2022

Same author

Development and Validation of a Mobile Application to Detect Visual Dysfunction Following Mild Traumatic Brain Injury.

Military medicine·2021

Same author

Genomic discovery of an evolutionarily programmed modality for small-molecule targeting of an intractable protein surface.

Proceedings of the National Academy of Sciences of the United States of America·2020

Same author

Activation of Ras in the Vascular Endothelium Induces Brain Vascular Malformations and Hemorrhagic Stroke.

Cell reports·2018

Same author

In vitro and in vivo DFO-chelatable labile iron release profiles among commercially available intravenous iron nanoparticle formulations.

Regulatory toxicology and pharmacology : RTP·2018

Same author

Plasma membrane temperature gradients and multiple cell permeabilization induced by low peak power density femtosecond lasers.

Biochemistry and biophysics reports·2017

Search research articles

Related Experiment Video

Updated: May 19, 2026

Laparoscopic Cholecystectomy with Indocyanine Green Fluorescence: Choledochoscopic Stone Extraction and Primary Duct Suture

Laparoscopic Cholecystectomy with Indocyanine Green Fluorescence: Choledochoscopic Stone Extraction and Primary Duct Suture

Published on: November 25, 2025

Dual-mode laparoscopic fluorescence image-guided surgery using a single camera.

Daniel C Gray¹, Evgenia M Kim, Victoria E Cotero

¹GE Global Research, One Research Circle, Niskayuna, NY, 12309, USA.

Biomedical Optics Express

|August 10, 2012

Summary

This summary is machine-generated.

This study introduces a dual-mode laparoscope for fluorescence image-guided surgery, aiding surgeons in visualizing nerves to prevent iatrogenic nerve damage during procedures.

Keywords:

(170.2150) Endoscopic imaging (170.3880) Medical and biological imaging (300.2530) Fluorescence, laser-induced

More Related Videos

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

Related Experiment Videos

Last Updated: May 19, 2026

Laparoscopic Cholecystectomy with Indocyanine Green Fluorescence: Choledochoscopic Stone Extraction and Primary Duct Suture

Laparoscopic Cholecystectomy with Indocyanine Green Fluorescence: Choledochoscopic Stone Extraction and Primary Duct Suture

Published on: November 25, 2025

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

Area of Science:

Surgical Technology
Medical Imaging
Neuroscience

Background:

Iatrogenic nerve damage is a significant surgical complication, leading to loss of function and chronic neuropathy.
Current visualization methods often fail to adequately identify nerves during surgery.
Fluorescence image-guided surgery presents a promising approach to mitigate intraoperative nerve injuries.

Purpose of the Study:

To develop and demonstrate a novel single-camera, dual-mode laparoscope for simultaneous white-light and fluorescence imaging.
To enhance intraoperative nerve visualization and reduce the risk of iatrogenic nerve damage.

Main Methods:

Development of a dual-mode laparoscope integrating white-light and fluorescence imaging capabilities.
Utilized a nerve-specific contrast agent for in situ rat nerve imaging.
Acquisition of full-color white-light and high-brightness fluorescence images and videos.

Main Results:

The dual-mode laparoscope successfully provided near simultaneous display of white-light and fluorescence nerve images.
Demonstrated visualization of various in situ rat nerves, including those as small as 100 µm in diameter.
High-quality fluorescence imaging highlighted nerve structures effectively.

Conclusions:

The developed dual-mode laparoscope is effective for fluorescence image-guided surgery.
This technology has the potential to significantly improve surgical safety by preventing nerve damage.
Further research may lead to broader clinical applications in various surgical fields.