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[Compare the composition and content of Arnebiae radix and the stem residues]
Jian Zhou1, Xue-Mei Tang, Cong Wang
1Chengdu University of TCM, Chengdu 610075, China. 47293315@qq.com
Objective:
To compare the composition and content of Arnebiae Radix and the stem residues.
Methods:
TLC and HPLC were used to identify Arnebia, ultraviolet-visible spectrophotometry was used to determine the content of hydroxy naphthoquinone total pigment in Arnebia, HPLC was used to determine the total content of /3-P'-dimethyl acrylamide Aka Ning and shikonin.
Results:
The number of spots of Arnebia Radix was consistent with that of the stem residues in 10 batches of medicinal materials, the former was larger and deeper in color. Their features of fingerprint are the same,at the same retention time,the peak area of radix was larger; The average content of hydroxy naphthoquinone total pigment was 3.631% in the radix, and 1.516% in the stem. The total content of beta-beta'-dimethyl acrylamide Aka Ning and shikonin in the radix and the stem were respectively 0.89% and 0.309%.
Conclusion:
The ingredients in the radix and the stem residues are the same, but the contents of root of the total pigment content of hydroxyl-naphthoquinone, beta-beta'-dimethyl acrylamide Aka Ning and shikonin are twice higher than those of the stem residues.
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