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Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

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Published on: February 19, 2020

Electrostatic-spray ionization mass spectrometry.

Liang Qiao¹, Romain Sartor, Natalia Gasilova

¹Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Analytical Chemistry

|August 11, 2012

Summary

This summary is machine-generated.

A novel electrostatic-spray ionization (ESTASI) method enables mass spectrometry (MS) analysis of samples on insulating substrates. This technique uses capacitive coupling for efficient ion generation, expanding MS applications.

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Area of Science:

Analytical Chemistry
Mass Spectrometry
Electrochemistry

Background:

Traditional mass spectrometry often requires conductive samples or specialized interfaces.
Analyzing samples on insulating substrates presents challenges for ionization and ion transfer.
Developing versatile ionization techniques is crucial for expanding mass spectrometry applications.

Purpose of the Study:

To introduce and characterize a new electrostatic-spray ionization (ESTASI) method for mass spectrometry.
To demonstrate the applicability of ESTASI across various sample formats and geometries.
To enable mass spectrometry analysis of samples deposited on insulating materials.

Main Methods:

Utilized electrostatic-spray ionization (ESTASI) via capacitive coupling between an electrode and the sample.
Applied high voltage pulses to induce electrostatic charging and bipolar ion spray.
Tested ESTASI with diverse sample presentations: silica capillaries, pipet tips, polymer microchannels, and droplets on polymer plates.
Integrated ESTASI with capillary electrophoresis fraction collection.

Main Results:

Successfully generated ions from samples on insulating substrates using ESTASI.
Demonstrated bipolar ion spray (cations followed by anions) with positive voltage pulses.
Showcased versatility by applying ESTASI to multiple geometries and sample types.
Confirmed ESTASI's compatibility with capillary electrophoresis for sequential analysis.

Conclusions:

ESTASI is an effective method for mass spectrometry analysis of samples on insulating substrates.
The capacitive coupling approach provides a robust ionization mechanism for diverse applications.
ESTASI broadens the scope of samples and formats amenable to mass spectrometry analysis.