Graphene enhanced electron transfer at aptamer modified electrode and its application in biosensing

Area of Science:

• Electrochemistry
• Materials Science
• Nanotechnology

Background:

• Graphene (GN) possesses unique properties for advanced applications.
• Electrochemical biosensors offer sensitive detection methods.
• Aptasensors utilize nucleic acid aptamers for target recognition.

Purpose of the Study:

• To develop a novel graphene-based electrochemical aptasensor for adenosine triphosphate (ATP) detection.
• To leverage the unique graphene/ssDNA interaction for biosensing.
• To establish a sensitive, selective, and label-free detection platform.

Main Methods:

• Immobilization of adenosine triphosphate binding aptamer (ABA) on a gold electrode.
• Utilizing graphene's electron transfer ability and GN/ssDNA interaction.
• Monitoring changes in charge transfer resistance (R(ct)) upon ATP binding.

Main Results:

• Graphene adsorption on ABA-modified electrodes decreased R(ct).
• ATP binding to ABA inhibited graphene adsorption, preventing R(ct) decrease.
• The aptasensor demonstrated high sensitivity and selectivity for ATP detection.
• Detection range for ATP was 15 × 10(-9) to 4 × 10(-3) M.

Conclusions:

• A label-free, graphene-based electrochemical aptasensor for ATP detection was successfully developed.
• The strategy shows promise for detecting other targets like Hg(2+) using specific oligonucleotides.
• This approach offers a versatile platform for high-performance electrochemical aptasensor design.