Related Experiment Video
Updated: May 19, 2026

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
Published on: April 5, 2015
Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based
Marina G Tarutina1, Tatiana A Dutova, Inna E Yezhova
1Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhny proezd 1, 117545 Moscow, Russia.
Abstract:
We identified Saccharomyces cerevisiae mutants with 100% higher intracellular glutathione using 1-methyl-3-nitro-1-nitrosoguanidine mutagenesis. This method employs visual selection of the most pigmented colonies among met30 strains carrying ade1 and ade2 mutations. Since the method does not involve genetic engineering, the mutants are suitable for use in the food industry.

