Related Experiment Video
Updated: May 19, 2026

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Complete genome sequence of a novel vitivirus isolated from grapevine
Maher Al Rwahnih1, Mysore R Sudarshana, Jerry K Uyemoto
1Department of Plant Pathology, University of California, Davis, California, USA.
Abstract:
A novel virus-like sequence from grapevine was identified by Illumina sequencing. The complete genome is 7,551 nucleotides in length, with polyadenylation at the 3' end. Translation of the sequence revealed five open reading frames (ORFs). The genomic organization was most similar to those of vitiviruses. The polymerase (ORF1) and coat protein (ORF4) genes shared 31 to 49% nucleotide and 40 to 70% amino acid sequence identities, respectively, with other grapevine vitiviruses. The virus was tentatively named grapevine virus F (GVF).
More Related Videos
08:56Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
Related Concept Videos
Viruses of Archaea
Viruses with RNA Genomes
Size and Structure of Viral Genomes
Human Virome
DNA Bacteriophages
Genomic DNA in Eukaryotes