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Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.

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Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
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Time-lapse cleavage rating predicts human embryo viability.

D Hlinka1, B Kaľatová, I Uhrinová

  • 1Fetal Medicine Program, Department of Cell Biology, Šafárik University, Košice, Slovak Republic.

Physiological Research
|August 14, 2012
PubMed
Summary
This summary is machine-generated.

Time-lapse embryo cleavage rating (ECR) precisely tracks mitotic events in human pre-implantation embryos. Deviations from normal timing or trichotomic mitosis predict poor development, enabling accurate identification of viable embryos.

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Last Updated: May 19, 2026

Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
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Published on: February 28, 2019

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
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Human Egg Maturity Assessment and Its Clinical Application
08:51

Human Egg Maturity Assessment and Its Clinical Application

Published on: August 19, 2019

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Embryology

Background:

  • Assessing human pre-implantation embryo viability is crucial for in vitro fertilization (IVF) success.
  • Conventional methods often fail to distinguish viable from non-viable embryos.
  • Time-lapse imaging offers a dynamic approach to monitor early embryonic development.

Purpose of the Study:

  • To analyze the chronological patterns of mitotic events in human pre-implantation embryos.
  • To determine the predictive value of cleavage timing and abnormal mitosis for embryo viability and developmental potential.
  • To evaluate time-lapse embryo cleavage rating (ECR) as a diagnostic tool in IVF.

Main Methods:

  • Utilized time-lapse imaging at 10-minute intervals to record mitotic events in human pre-implantation embryos.
  • Quantified durations of interphases (i) and cleavage clusters (c) for zygotes and early cleavage stages.
  • Identified and characterized cases of trichotomic mitosis, where embryos divided into an abnormal number of cells.

Main Results:

  • Established uniform time-patterning for mitotic events in viable zygotes and blastocysts (e.g., i2=11±1h, c2=15±5min).
  • Demonstrated that deviations in cell cycle durations strongly predict poor implantation and development.
  • Identified trichotomic mitosis in 17% of cases, with these embryos proving unviable despite appearing normal conventionally.
  • ECR demonstrated 90% specificity in identifying viable embryos and 100% specificity in eliminating unviable ones.

Conclusions:

  • Time-lapse embryo cleavage rating (ECR) is a highly specific, non-invasive method for assessing early embryo viability.
  • Abnormal mitotic timing and trichotomic mitosis are critical indicators of embryo non-viability.
  • ECR can significantly improve embryo selection in clinical IVF programs, reducing the transfer of non-viable embryos.