Participation of haemocytes in fat body degradation via cathepsin L expression

Insect haemocytes are known to participate in innate immunity via the phagocytosis of pathogens. However, the function of haemocytes in tissue remodelling is less understood. We report here that haemocytes play roles in fat body degradation by expressing a cysteine proteinase cathepsin L in the lepidopteran Helicoverpa armigera. During metamorphosis, haemocytes undergo morphological changes by increasing their cell size and transforming their granulocytes into macrogranulocytes. The population of haemocytes also changes with increased number of granulocytes and decreased plasmatocytes. The expression level of cathepsin L in haemocytes, mainly in granulocytes and plasmatocytes, increases. The steroid hormone 20-hydroxyecdysone is able to promote the transformation of granulocytes into macrogranulocytes, and up-regulate the expression level of cathepsin L. The knock-down of the cathepsin L gene by RNA interference in haemocytes in vitro results in deficient granulocytes transforming into macrogranulocytes. Haemocytes are able to enter the decomposed fat body during metamorphosis. The over-expression of the proteinase domain C1A of cathepsin L results in cell apoptosis. Haemocytes, especially macrogranulocytes, undergo apoptosis and cathepsin L is released into haemolymph and the fat body during metamorphosis for fat body decomposition and degradation. These results suggest that cathepsin L is related to the transformation of granulocytes to macrogranulocytes to enter the fat body, and induce haemocyte apoptosis for further tissue degradation.