Related Experiment Video
Updated: May 19, 2026

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
Published on: May 11, 2020
Louis Pasteur, from crystals of life to vaccination
1Hôpital Necker-Enfants malades, Faculté de médecine Paris Descartes, Paris, France. patrick.berche@gmail.com
Abstract:
Louis Pasteur (1822-1895) is an exceptional scientist who opened a new era in medicine and biology. Starting from studies on crystals of by-products of wine fermentation, he first defined a distinct chemistry between dead and living matters. He then showed the role of living microbes in the fermentation and putrefaction processes. This brought him to challenge the two-millennium-old theory of spontaneous generation, using remarkably well-designed experiments. His observations on epidemics in silkworms allowed him to demonstrate the role of specific germs in infectious diseases. His discovery of the vaccine against fowl cholera can be considered as the birth of immunology. Finally, he became universally recognized through his famous vaccinations against anthrax and rabies.
Related Concept Videos
History of Microbiology
Vaccinations
Vaccine Production
Microorganisms in Medicine and Therapeutics
Vaccines
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

