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Related Experiment Video

Updated: May 19, 2026

Quantitation of Rabies Virus in Various Bovine Brain Structures
13:42

Quantitation of Rabies Virus in Various Bovine Brain Structures

Published on: May 22, 2021

[Identification and sequential analysis on rabies virus isolated from a donkey].

Ting-bo Xie1, Hua Yu, Jie Wu

  • 1Center for Rabies Diagnosis, Wuhan Institute of Biological Products, Wuhan 430060, China.

Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi
|August 14, 2012
PubMed
Summary

A novel rabies virus (RABV) was identified in a donkey in Wuhan. Genetic analysis revealed its close relationship to local street strains and vaccine strains, aiding rabies virus surveillance.

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Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology
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Published on: August 18, 2023

Related Experiment Videos

Last Updated: May 19, 2026

Quantitation of Rabies Virus in Various Bovine Brain Structures
13:42

Quantitation of Rabies Virus in Various Bovine Brain Structures

Published on: May 22, 2021

Postmortem Diagnosis of Rabies in Animals by the Updated, Multiplexed LN34 Real-Time Reverse Transcription-Polymerase Chain Reaction Assay
09:54

Postmortem Diagnosis of Rabies in Animals by the Updated, Multiplexed LN34 Real-Time Reverse Transcription-Polymerase Chain Reaction Assay

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Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology
10:26

Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology

Published on: August 18, 2023

Area of Science:

  • Veterinary Virology
  • Molecular Epidemiology
  • Zoonotic Diseases

Context:

  • Rabies virus (RABV) poses a significant public health threat.
  • Animal rabies cases require continuous genetic surveillance to understand viral evolution and transmission dynamics.
  • Donkey rabies cases are infrequently reported, necessitating detailed genetic characterization.

Purpose:

  • To identify and genetically characterize the nucleoprotein (N) and glycoprotein (G) genes of a RABV strain isolated from a donkey in Wuhan.
  • To compare the genetic makeup of the isolated RABV with regional street strains and global vaccine strains.
  • To assess the phylogenetic relationship of the novel RABV strain.

Summary:

  • Rabies virus was detected in a donkey and subsequently in experimentally infected suckling mice.
  • Nucleotide and amino acid homology analysis of the N and G genes showed high similarity (85.7%-99.7%) to various street and vaccine strains.
  • The isolated RABV demonstrated a close genetic relationship to representative street strains in Hubei and vaccine strains used in China and internationally.

Impact:

  • Successful isolation and genetic identification of a novel RABV strain from an unusual host (donkey).
  • Provides crucial genetic data for rabies virus surveillance and control strategies in the region.
  • Enhances understanding of RABV diversity and potential implications for vaccine efficacy.