Related Experiment Video
Updated: May 19, 2026
Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats
Published on: December 5, 2017
[Vasodilator effect of oroxylin A on thoracic aorta isolated from rats]
Hong Wang1, Jing-Tian Qu, Xin Zhao
1Tianjin State Key Laboratory of Modern Chinese Medicine, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, China. wanghongsys@126.com
Objective:
To investigate the vasodilator effect and the endothelium-dependent mechanism of oroxylin A in thoracic aorta isolated from rats.
Methods:
Thoracic aorta was isolated from Wistar rats. After pretreatment with norepinephrine or KCl, the effects of oroxylin A at different concentrations were detected on isolated vascular rings prepared from rats' thoracic aorta. The response of thoracic aortic ring was evaluated in the presence and absence of endothelium, and NG-nitro-L-arginine methyl ester (L-NAME), a specific inhibitor of nitric oxide synthase.
Results:
Oroxylin A (10 and 100 μmol/L) caused vasodilation on endothelium-intact aortic rings pretreated with norepinephrine (1 μmol/L) and KCl (60 mmol/L) compared with the control (P<0.05, P<0.01). The vasodilation function of 10 and 100 μmol/L oroxylin A on the endothelium-denuded aorta rings was significantly lower than that on the endothelium-intact aorta rings (P<0.05, P<0.01). L-NAME pretreatment significantly attenuated the effect of 100 μmol/L oroxylin A on endothelium-intact aorta rings (P<0.05, P<0.01).
Conclusion:
Oroxylin A can induce the relaxation of the aorta ring in endothelium-dependent manner. Nitric oxide may be involved in the endothelium-dependent effect of oroxylin A.
