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Updated: May 19, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
[Construction of a red fluorescent shuttle vector controlled by recA operon promoter of Streptococcus mutans]
Wen-ming Huang1, Yang-long Xu, De-qin Yang
1Department of Oral Medicine, The Affiliated Hospital of Stomatology, Zunyi Medical College, Zunyi Guizhou 563003, China.
Objective:
To construct a red fluorescent shuttle vector controlled by recA operon promoter to transform Streptococcus mutans.
Methods:
The promoter of recA was amplified from Streptococcus mutans UA159, and connected to plasmid pDsRed2-N1 to construct pRred with a red fluorescent coding gene, which was then inserted into the shuttle vector pDL276 to construct pLRred.
Results:
pLRred was successfully constructed, and Escherichia coli transformed with the pLRred plasmid could express reporter gene DsRed.
Conclusions:
The recombination plasmid pLRred can be used in the further research of the expression of cariogenic virulence factor gene by Streptococcus mutans in biofilm.
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