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Related Experiment Video

Updated: May 19, 2026

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
12:22

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

Published on: April 2, 2012

Factors affecting herpes simplex virus reactivation from the explanted mouse brain.

Hui-Wen Yao1, Pin Ling, Shih-Heng Chen

  • 1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan 701, Republic of China.

Virology
|August 14, 2012
PubMed
Summary

Herpes simplex virus type I (HSV-1) reactivation in the brain is linked to viral virulence and host susceptibility. A new explant assay reveals factors influencing HSV-1 reactivation from latency.

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Published on: August 24, 2015

Area of Science:

  • Neurovirology
  • Immunology

Background:

  • Herpes simplex virus type I (HSV-1) encephalitis often results from viral reactivation from latency.
  • Factors influencing HSV-1 reactivation in the brain remain poorly understood due to a lack of sensitive detection methods.

Purpose of the Study:

  • To establish and utilize a novel ex vivo explant assay to investigate factors influencing HSV-1 reactivation in the mouse brain.
  • To examine the impact of HSV-1 strain neurovirulence and mouse host susceptibility on viral reactivation.

Main Methods:

  • Developed an ex vivo explant assay using dissociated mouse brain tissue to induce HSV-1 reactivation.
  • Infected three different mouse strains with four HSV-1 strains of varying neurovirulence.
  • Assessed viral growth during acute infection, latent viral genome load, and explant reactivation efficiency.

Main Results:

  • Virulent HSV-1 strains and susceptible mouse strains showed prolonged viral growth and increased latent viral genomes.
  • Efficient explant reactivation was observed in the brain stem of susceptible mice infected with virulent strains.
  • Both viral neurovirulence and host susceptibility positively correlated with HSV-1 reactivation.

Conclusions:

  • The established ex vivo explant assay is effective for studying HSV-1 reactivation in the brain.
  • Viral neurovirulence and host susceptibility are key determinants of HSV-1 reactivation from latency in the mouse brain.
  • Findings provide insights into the pathogenesis of HSV-1 encephalitis and potential therapeutic targets.