Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Time-resolved fluorescence in biospecific assays.

E Soini1, I Hemmilä, P Dahlén

  • 1Wallac Oy, Turku, Finland.

Annales De Biologie Clinique
|January 1, 1990
PubMed
Summary

Time-resolved fluorometry with lanthanide chelates offers high sensitivity for immunoassays and DNA hybridization assays. These chelates are a versatile alternative to organic dyes in microscopy and multiparameter assays.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Relationship of faecal calprotectin and long-term outcomes in Finnish patients with Crohn's disease: retrospective multi-centre chart review study.

Scandinavian journal of gastroenterology·2019
Same author

Spinach consumption ameliorates the gut microbiota and dislipaemia in rats with diet-induced non-alcoholic fatty liver disease (NAFLD).

Food & function·2019
Same author

A study of the prebiotic-like effects of tomato juice consumption in rats with diet-induced non-alcoholic fatty liver disease (NAFLD).

Food & function·2017
Same author

Quantitative Assessment of Steroid Hormone Binding Sites by Thaw-Mount Autoradiography <sup>1</sup> , <sup>2</sup>.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2017
Same author

Cost-Utility Analysis (cua) Of First-Line Disease-Modifying Treatments (DMT) Versus Best Supportive Care (Bsc) In Finnish Relapsing-Remitting Multiple Sclerosis (RRMS) Patients.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
Same author

Modelling the Persistence of Disease-Modifying Drug Treatment (DMT) and its Independent Drivers in Finnish Multiple Sclerosis (MS) Patients: Parametric Survival Modelling.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Fluorescent dyes are crucial for bio-assays, but limitations exist.
  • Lanthanide chelates offer unique fluorescent properties.
  • Time-resolved fluorometry enhances detection sensitivity.

Purpose of the Study:

  • To review fluorescent lanthanide chelates and their detection via time-resolved fluorometry.
  • To compare their sensitivity with other fluorescent dyes and labelling materials.
  • To assess their utility in immunoassays, DNA assays, and microscopy.

Main Methods:

  • Review of literature on lanthanide chelates and time-resolved fluorometry.
  • Discussion of minimum detectable doses and theoretical sensitivities.
  • Comparison of experimental results with theoretical limits.

Main Results:

  • Time-resolved fluorometry with lanthanide chelates provides high sensitivity in immunoassays.
  • Sensitivity in DNA hybridization assays is comparable to phosphorus-32.
  • Lanthanide chelates are a viable alternative to organic fluorescent dyes in microscopy.
  • Lanthanide chelates are particularly useful in multiparameter assays.

Conclusions:

  • Lanthanide chelates combined with time-resolved fluorometry are highly sensitive bio-assay tools.
  • They offer advantages over traditional organic dyes, especially in complex assays.
  • The combination of organic dyes and lanthanide chelates maximizes assay parameter separation.

Related Experiment Videos