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Cytoskeletal Coordination in Cell Migration

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A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...

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Related Experiment Video

Updated: May 19, 2026

Nuclear Migration in the Drosophila Oocyte

Nuclear Migration in the Drosophila Oocyte

Published on: May 13, 2021

Apical movement during interkinetic nuclear migration is a two-step process.

Philip C Spear¹, Carol A Erickson

¹Department of Molecular and Cellular Biology, One Shields Ave., UC Davis, Davis, CA 95616, United States. pcspear@ucdavis.edu

Developmental Biology

|August 14, 2012

Summary

This summary is machine-generated.

Neural progenitor cells migrate their nuclei apically during G2 phase using microtubules. Mitosis then occurs at the apical surface through an actin-dependent rounding process, revealing a new model for interkinetic nuclear migration.

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Last Updated: May 19, 2026

Nuclear Migration in the Drosophila Oocyte

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Published on: May 13, 2021

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Capturing Cytoskeleton-Based Agitation of the Mouse Oocyte Nucleus Across Spatial Scales

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Published on: January 12, 2024

Area of Science:

Developmental Biology
Cell Biology
Neuroscience

Background:

Neural progenitor cells in vertebrate neuroepithelium exhibit interkinetic nuclear migration (INM).
Mitotic cells are typically found at the apical surface, while interphase nuclei are distributed basally.
The precise mechanisms of nuclear apical migration and mitosis coordination remain unclear.

Purpose of the Study:

To elucidate the mechanisms underlying nuclear migration during interkinetic nuclear migration.
To understand how mitosis is coordinated with nuclear positioning in neural progenitor cells.
To propose a revised model for interkinetic nuclear migration.

Main Methods:

Time-lapse confocal microscopy was employed to observe nuclear and centrosome dynamics.
Experiments were conducted using chicken neural tube and mouse cortical slice preparations.

Main Results:

Nuclei migrate apically in G2 phase, facilitated by microtubules.
Centrosomes detach from the apical surface late in G2, initiating mitosis away from the apex.
The mitotic cell rounds up to the apical surface via an actin-dependent mechanism.

Conclusions:

Interkinetic nuclear migration involves a complex interplay between microtubules and actin.
A novel model is proposed where microtubules guide nuclear migration and actin facilitates apical rounding for mitosis.
This mechanism is conserved across different vertebrate species.