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Yeast one-hybrid assays: a historical and technical perspective

John S Reece-Hoyes1, A J Marian Walhout

  • 1Program in Systems Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA. john.reece-hoyes@umassmed.edu

Methods (San Diego, Calif.)
|August 14, 2012
PubMed

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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...