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Site-specific labeling of his-tagged Nanobodies with ⁹⁹mTc: a practical guide

Catarina Xavier1, Nick Devoogdt, Sophie Hernot

  • 1In Vivo Cellular and Molecular Imaging (ICMI) Laboratory, Vrije Universiteit Brussel (VUB), Brussels, Belgium.

Methods in Molecular Biology (Clifton, N.J.)
|August 14, 2012
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...