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Updated: May 19, 2026
04:20
Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity
Published on: February 9, 2024
FAM83B mediates EGFR- and RAS-driven oncogenic transformation
Rocky Cipriano1, James Graham, Kristy L S Miskimen
1Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA.
The Journal of Clinical Investigation
|August 14, 2012
Summary
Family with sequence similarity 83, member B (FAM83B) acts as an oncogene by activating MAPK and mTOR signaling pathways. FAM83B is overexpressed in cancers, correlating with poor survival, suggesting it as a therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Signaling Pathways
Background:
- Aberrant growth signaling drives cancer development, often via activated growth factor receptors or downstream pathways.
- RAS pathway activation is a common mechanism in tumorigenesis.
Purpose of the Study:
- To identify novel mediators of RAS-driven transformation.
- To investigate the role of FAM83B in cancer signaling and its therapeutic potential.
Main Methods:
- Validation-based insertional mutagenesis (VBIM) to identify FAM83B.
- Co-precipitation assays to study FAM83B-CRAF interaction.
- Cell proliferation and malignant phenotype assays.
- Analysis of FAM83B expression in human tumor specimens.
Main Results:
- FAM83B substitutes for RAS in transforming human mammary epithelial cells (HMECs).
- FAM83B binds CRAF, disrupting 14-3-3 interactions and enhancing MAPK/mTOR signaling.
- FAM83B ablation inhibits tumor cell proliferation and malignant phenotype.
- Elevated FAM83B expression in human cancers correlates with subtypes, grade, and reduced survival.
Conclusions:
- FAM83B is a key intermediary in EGFR/RAS/MAPK signaling and functions as an oncogene.
- FAM83B represents a potential therapeutic target for cancer treatment.
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