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TRAF6 regulates proliferation, apoptosis, and invasion of osteosarcoma cell

Qingbing Meng1, Minqian Zheng, Hongbing Liu

  • 1Orthopedics Department, Yancheng City No. 1 People's Hospital, 16 Yue-He Road, Yancheng, Jiangsu 224005, People's Republic of China. ycmengqingbing@163.com

Molecular and Cellular Biochemistry
|August 14, 2012
PubMed

Insights

Tumor necrosis factor receptor-associated factor 6 (TRAF6) is overexpressed in osteosarcoma, correlating with tumor progression and metastasis. Reducing TRAF6 expression inhibits osteosarcoma cell invasion and proliferation, suggesting its role in tumorigenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • TRAF6 is a key signaling mediator for TNF and IL-1 receptor superfamilies.
  • TRAF6 is implicated in tumorigenesis, invasion, and metastasis.

Purpose of the Study:

  • To investigate TRAF6 expression in osteosarcoma tissues.
  • To correlate TRAF6 expression with clinical pathology and invasion.
  • To explore TRAF6's role in osteosarcoma cell behavior.

Main Methods:

  • RT-PCR and Western blot to assess TRAF6 mRNA and protein expression.
  • RNA interference (siRNA) to down-regulate TRAF6 in MG-63 cells.
  • Transwell assays to evaluate cell invasion and proliferation.
  • Apoptosis assays to measure programmed cell death.

Main Results:

  • TRAF6 mRNA and protein were significantly upregulated in osteosarcoma tissues, especially with lung metastasis and higher Enneking stage.
  • Down-regulation of TRAF6 in MG-63 cells reduced proliferation and invasion, and increased apoptosis.
  • TRAF6 knockdown decreased p-p65, cyclin D1, and MMP-9, while increasing caspase 3 expression.

Conclusions:

  • TRAF6 overexpression is associated with osteosarcoma tumorigenesis and invasion.
  • TRAF6 plays a critical role in regulating osteosarcoma cell proliferation, invasion, and apoptosis.
  • TRAF6 may serve as a potential therapeutic target for osteosarcoma.

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