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Second round robin for plasma hepcidin methods: first steps toward harmonization.

Joyce J C Kroot1, Antonius E van Herwaarden, Harold Tjalsma

  • 1Department of Laboratory Medicine, Laboratory of Genetic Endocrine and Metabolic Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.

American Journal of Hematology
|August 14, 2012
PubMed
Summary
This summary is machine-generated.

Harmonizing plasma hepcidin measurements is crucial for consistent results. This study developed algorithms to calculate hepcidin consensus (HEPCON) values, improving agreement between different laboratory methods.

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Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Hormone Assays

Background:

  • Hepcidin, the iron regulatory hormone, exhibits significant variability in measurements across different laboratories and methodologies.
  • This lack of harmonization leads to inconsistent numerical results for the same clinical samples, hindering research and clinical interpretation.

Purpose of the Study:

  • To investigate the feasibility of harmonizing plasma hepcidin measurements through method standardization.
  • To develop a reliable method for achieving better agreement between diverse hepcidin assay techniques.

Main Methods:

  • Distribution of native plasma pools and synthetic hepcidin-25 samples to 21 global methods for comparative analysis.
  • Assessment of sample commutability using Bland-Altman plots to compare synthetic and native hepcidin results.
  • Development of algorithms based on consensus values from mutually agreeing native hepcidin methods.

Main Results:

  • Significant differences in absolute values and reproducibility were observed among the 21 hepcidin measurement methods.
  • Synthetic hepcidin-25 samples were found to be non-commutable with native samples for most methods.
  • Developed algorithms substantially reduced the between-method coefficient of variation (CV) for hepcidin quantification.

Conclusions:

  • Hepcidin harmonization can be achieved using specific algorithms to calculate hepcidin consensus (HEPCON) values, even in the absence of fully commutable materials.
  • These algorithms enable laboratories to report hepcidin concentrations in a standardized HEPCON format, facilitating data aggregation across studies.
  • This work represents a significant first step towards harmonized plasma hepcidin measurements, improving data comparability in research and clinical settings.