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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
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Published on: March 17, 2023

Separation technologies for stem cell bioprocessing.

Maria Margarida Diogo1, Cláudia Lobato da Silva, Joaquim M S Cabral

  • 1Department of Bioengineering and Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Technical University of Lisbon, Lisbon, Portugal.

Biotechnology and Bioengineering
|August 14, 2012
PubMed
Summary
This summary is machine-generated.

Efficient stem cell separation is vital for regenerative medicine and disease research. This review assesses current and emerging techniques, emphasizing high-resolution, cost-effective methods for isolating diverse stem cell populations.

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Area of Science:

  • Stem cell biology and regenerative medicine.
  • Biophysical and affinity-based separation technologies.
  • Cellular therapies and drug discovery.

Background:

  • Stem cells hold significant promise for regenerative medicine, drug discovery, and understanding disease mechanisms.
  • Effective isolation and purification of stem cells are critical for realizing their full therapeutic and research potential.
  • Existing separation methods, while useful for hematopoietic stem cells, face challenges in scalability, resolution, and cost for newer stem cell applications.

Purpose of the Study:

  • To critically review existing and emerging separation processes for stem cells.
  • To highlight the need for novel, high-resolution, and cost-effective separation techniques.
  • To explore applications in isolating heterogeneous stem cell populations and depleting tumorigenic cells.

Main Methods:

  • Categorization of separation techniques based on physical, biophysical, and affinity properties.
  • Assessment of traditional and advanced cell separation methods.
  • Focus on novel affinity-based methods using aptamers and lectins.
  • Emphasis on label-free biophysical methods integrated with microscale technologies.

Main Results:

  • Current separation methods require enhancement for resolution, scalability, and cost-effectiveness.
  • Novel affinity-based approaches offer improved specificity for stem cell isolation.
  • Label-free biophysical methods, especially microscale technologies, present promising avenues for efficient separation.
  • These advancements are crucial for studying stem cell heterogeneity and ensuring therapeutic safety.

Conclusions:

  • Advanced separation technologies are essential for unlocking the full potential of stem cells in research and therapy.
  • Future directions include developing more sophisticated affinity ligands and integrated microfluidic systems.
  • Optimized stem cell separation will accelerate progress in regenerative medicine, disease modeling, and drug development.