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Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: May 19, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Stepping stones in DNA sequencing.

Henrik Stranneheim1, Joakim Lundeberg

  • 1Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden. henrik.stranneheim@scilifelab.se

Biotechnology Journal
|August 14, 2012
PubMed
Summary
This summary is machine-generated.

Advances in DNA sequencing technology allow for faster, cheaper genetic analysis. Nanopore sequencing promises longer DNA reads, simplifying data handling and expanding genomic research possibilities.

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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Published on: July 10, 2019

Related Experiment Videos

Last Updated: May 19, 2026

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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Published on: July 10, 2019

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Rapid advancements in DNA sequencing have revolutionized genetics and biology.
  • Massive amounts of sequence data present challenges in pre-sequencing and downstream analysis.
  • Traditional sequencing methods are limited to short DNA fragments (approx. 1000 bases).

Purpose of the Study:

  • To review technical advances in DNA sequencing.
  • To highlight innovations addressing read-length limitations.
  • To explore new frontiers opened in genomics.

Main Methods:

  • Review of recent technological breakthroughs in DNA sequencing.
  • Discussion of nanopore sequencing technology.
  • Analysis of challenges in handling large-scale sequence data.

Main Results:

  • Nanopore sequencing offers the potential for sequencing long, intact DNA fragments.
  • This technology promises to overcome current read-length limitations.
  • Improved accuracy and longer reads simplify downstream analysis.

Conclusions:

  • Nanopore sequencing represents a significant advancement in genomics.
  • The ability to sequence longer DNA fragments enhances analytical power.
  • These advances are crucial for deeper understanding of molecular events in life processes.