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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Related Experiment Video

Updated: May 19, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

On-line CE/ESI/MS interfacing: recent developments and applications in proteomics.

Jana Krenkova1, Frantisek Foret

  • 1Institute of Analytical Chemistry of the ASCR, Brno, Czech Republic. krenkova@iach.cz

Proteomics
|August 14, 2012
PubMed
Summary
This summary is machine-generated.

Capillary electrophoresis (CE) is regaining popularity in proteomics and metabolomics, complementing liquid chromatography. Recent advancements focus on online CE interfacing with mass spectrometry for pharmaceutical and biological analyses.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Separation Science

Background:

  • Capillary electrophoresis (CE) was pivotal for the Human Genome Project but saw reduced use in the early 2000s.
  • Renewed interest in CE stems from its complementary role in proteomics, metabolomics, and glycomics, alongside liquid chromatography.
  • Regulatory demands for additional separation techniques in pharmaceutical characterization are driving CE adoption.

Purpose of the Study:

  • To provide an overview of recent developments in online CE interfacing with electrospray ionization/mass spectrometry (ESI/MS).
  • To discuss instrumentation and applications of CE/ESI/MS in analyzing peptides, proteins, and glycans, focusing on the last three years.
  • To include related techniques like sample preconcentration, on-line protein digestion, and analyte derivatization for CE/ESI/MS.

Main Methods:

  • Review of recent literature (past 3 years) on CE/ESI/MS instrumentation and applications.
  • Focus on online interfacing techniques for seamless coupling of CE separation with ESI/MS detection.
  • Inclusion of sample preparation techniques relevant to CE/ESI/MS analysis.

Main Results:

  • Recent advancements in instrumentation have enhanced the online interfacing of CE with ESI/MS.
  • Successful applications demonstrated for the analysis of peptides, proteins, and glycans.
  • Integration of sample preconcentration, on-line digestion, and derivatization improves CE/ESI/MS capabilities.

Conclusions:

  • Online CE/ESI/MS is a powerful hyphenated technique with growing importance in biological and pharmaceutical analysis.
  • The reviewed developments highlight the technique's versatility and effectiveness in complex sample analyses.
  • CE/ESI/MS, particularly with integrated sample handling, offers significant advantages for proteomics and glycomics.