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Related Experiment Videos

K(3)EDTA Vacuum Tubes Validation for Routine Hematological Testing.

Gabriel Lima-Oliveira1, Giuseppe Lippi, Gian Luca Salvagno

  • 1Laboratory of Clinical Biochemistry, Department of Life and Reproduction Sciences, University of Verona, 37129 Verona, Italy.

ISRN Hematology
|August 14, 2012
PubMed
Summary
This summary is machine-generated.

This study validated two brands of K(3)EDTA vacuum tubes for hematological testing. Results showed brand differences significantly impacted only mean platelet volume (MPV) and platelet distribution width (PDW).

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Area of Science:

  • Clinical Chemistry
  • Hematology
  • In Vitro Diagnostics

Background:

  • In vitro diagnostic devices, such as blood collection vacuum tubes, are often used without prior validation.
  • Laboratory and hospital managers frequently select vacuum tubes based on cost or brand preference, potentially impacting test accuracy.

Purpose of the Study:

  • To validate two brands of dry K(3)EDTA vacuum tubes for routine hematological testing.
  • To assess the impact of different vacuum tube brands on hematological test results.

Main Methods:

  • Blood specimens from 100 volunteers were collected using two different K(3)EDTA vacuum tubes.
  • Hematological testing was performed on the Advia 2120i hematology system.
  • Paired Student's t-test was used to assess the significance of differences (P < 0.05).

Main Results:

  • Significant variations between the two evaluated K(3)EDTA vacuum tube brands were observed.
  • These variations were clinically relevant specifically for mean platelet volume (MPV) and platelet distribution width (PDW).

Conclusions:

  • The brand of K(3)EDTA vacuum tubes can be a source of clinically relevant variation for specific hematological parameters.
  • Laboratory managers can use this validation data to select vacuum tubes based on technical or economic factors for routine hematological tests.