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Related Experiment Video

Updated: May 19, 2026

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
09:33

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Published on: October 3, 2019

A stable cranial neural crest cell line from mouse.

Mamoru Ishii1, Athena C Arias, Liqiong Liu

  • 1Department of Biochemistry and Molecular Biology, USC/Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.

Stem Cells and Development
|August 15, 2012
PubMed
Summary

Researchers developed a method to culture cranial neural crest cells as multipotent stem-like cells. The O9-1 cell line represents a valuable tool for studying cranial neural crest development and differentiation.

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Craniofacial Development

Background:

  • Cranial neural crest cells form diverse craniofacial structures.
  • Previously, multipotent cranial neural crest stem cells were thought to be transient and undergo early lineage restriction.

Purpose of the Study:

  • To develop culture conditions enabling the indefinite propagation of multipotent cranial neural crest stem-like cells.
  • To characterize a novel cell line derived from these cultures for studying cranial neural crest development.

Main Methods:

  • Established culture conditions for cranial neural crest cells.
  • Derived and characterized two independent cell lines (O9-1 and i10-1).
  • Performed whole-genome expression profiling and in vivo differentiation assays.

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Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
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Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Published on: June 2, 2012

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay
08:55

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay

Published on: February 14, 2025

Related Experiment Videos

Last Updated: May 19, 2026

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
09:33

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Published on: October 3, 2019

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
12:48

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Published on: June 2, 2012

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay
08:55

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay

Published on: February 14, 2025

Main Results:

  • Developed culture conditions allowing cranial neural crest cells to be grown as multipotent stem-like cells.
  • Established the O9-1 cell line, which can be propagated indefinitely and differentiates into osteoblasts, chondrocytes, smooth muscle cells, and glial cells.
  • O9-1 cells express key stem cell and neural crest markers and contribute to cranial mesenchymal fates in vivo.

Conclusions:

  • The O9-1 cell line represents multipotent mesenchymal cranial neural crest cells.
  • This cell line is a valuable tool for investigating the molecular mechanisms of cranial neural crest differentiation.