Related Experiment Video

Updated: May 19, 2026

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

Protein analyses using differential ion mobility microchips with mass spectrometry

Alexandre A Shvartsburg, Richard D Smith

    Analytical Chemistry
    |August 15, 2012
    PubMed

    More Related Videos

    Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments08:40

    Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

    Published on: January 20, 2022

    Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry05:52

    Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry

    Published on: January 12, 2024

    Related Concept Videos

    Mass Spectrometry: Complex Analysis01:21

    Mass Spectrometry: Complex Analysis

    Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
    GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
    Tandem Mass Spectrometry01:21

    Tandem Mass Spectrometry

    Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
    Peptide Identification Using Tandem Mass Spectrometry01:33

    Peptide Identification Using Tandem Mass Spectrometry

    Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
    This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
    MALDI-TOF Mass Spectrometry01:19

    MALDI-TOF Mass Spectrometry

    Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
    Electrospray Ionization (ESI) Mass Spectrometry01:12

    Electrospray Ionization (ESI) Mass Spectrometry

    Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
    ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
    Mass Spectrometers01:16

    Mass Spectrometers

    This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions: