Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lactoferrin binding molecules in human seminal plasma.

C J Thaler1, O A Vanderpuye, J A McIntyre

  • 1Center for Reproduction and Transplantation Immunology, Methodist Hospital of Indiana, Inc. Indianapolis, Indiana 46202.

Biology of Reproduction
|October 1, 1990
PubMed
Summary

Seminal plasma contains molecules that bind to lactoferrin, altering its properties. This interaction may influence lactoferrin's biological activity and sperm binding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Where have we come from and where are we going? The paediatric HIV programme in Johannesburg, South Africa, from 2004 to 2018: A retrospective analysis of programme trends.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2022
Same author

Effect of COVID-19 on HIV, tuberculosis, and prevention of mother-to-child transmission of HIV indicators in Mopani district, South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2021
Same author

Primary healthcare workers at risk during COVID-19: An analysis of infections in HIV service providers in five districts of South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2021
Same author

HIV self-screening distribution preferences and experiences among men who have sex with men in Mpumalanga Province: Informing policy for South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2019
Same author

Provision of HIV services by community health workers should be strengthened to achieve full programme potential: a cross-sectional analysis in rural South Africa.

Tropical medicine & international health : TM & IH·2019
Same author

Burden of tuberculosis in HIV-positive pregnant women in Cape Town, South Africa.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2018

Area of Science:

  • Reproductive Biology
  • Biochemistry
  • Immunology

Background:

  • Lactoferrin, an iron-binding protein, interacts with sperm during ejaculation.
  • The properties of lactoferrin in seminal plasma (SP) differ from purified lactoferrin, suggesting interactions with other seminal molecules.
  • These differences may impact lactoferrin's biological functions.

Purpose of the Study:

  • To identify and characterize lactoferrin-binding molecules in seminal plasma.
  • To investigate how these interactions affect lactoferrin's properties and potential functions.

Main Methods:

  • Microtiter plate assays to detect lactoferrin binding in SP.
  • Biochemical characterization using SDS-PAGE and ligand blotting.
  • Crossed immunoelectrophoresis to study lactoferrin association in SP.

Related Experiment Videos

Main Results:

  • Seminal plasma specifically binds biotinylated lactoferrin.
  • Lactoferrin binding molecules in SP have approximate molecular weights of 120, 60, and 30 kDa.
  • Lactoferrin in SP exists in both free and bound forms, indicating association with other molecules.

Conclusions:

  • Seminal plasma contains specific lactoferrin-binding molecules.
  • These interactions alter lactoferrin's physicochemical properties.
  • Such alterations could influence lactoferrin's biological activity and sperm-binding affinity.