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Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Published on: August 31, 2016

Quantifying hematopoietic stem and progenitor cell mobilization.

Shiri Gur-Cohen¹, Kfir Lapid, Tsvee Lapidot

¹Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.

Methods in Molecular Biology (Clifton, N.J.)

|August 15, 2012

Summary

This summary is machine-generated.

Quantifying mobilized hematopoietic stem and progenitor cells (HSPC) is crucial for transplantation success. This chapter details methods to assess HSPC number and function in circulation for both human and murine models.

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Area of Science:

Hematology
Stem Cell Biology
Transplantation Medicine

Background:

Allogeneic donor blood cells and autologous peripheral blood leukocytes (PBL) are primary sources of hematopoietic stem and progenitor cells (HSPC) for transplantation.
Mobilization procedures are employed to increase circulating HSPC numbers.
Assessing the quantity and functional capacity of mobilized HSPC is vital for predicting transplantation outcomes.

Purpose of the Study:

To describe quantitative methods for measuring mobilized HSPC.
To evaluate the functional properties of mobilized HSPC in vitro and in vivo.
To correlate HSPC mobilization with long-term hematopoietic reconstitution capacity.

Main Methods:

Quantitative assays to determine the number of mobilized HSPC based on specific criteria.
In vitro functional assays to assess progenitor cell properties.
In vivo assays to evaluate hematopoietic reconstitution capacity.
Methods applicable to both human and murine HSPC mobilization.

Main Results:

The chapter provides detailed methodologies for HSPC quantification.
Functional assessments of mobilized HSPC are described.
The described assays enable correlation of mobilization extent with reconstitution potential.

Conclusions:

Accurate quantification and functional assessment of mobilized HSPC are essential for successful transplantation.
The described methods offer a comprehensive approach to evaluating HSPC mobilization in preclinical and clinical settings.
These assays are valuable tools for research in stem cell mobilization and transplantation.