Potential role of tRNAs in wheat and Lolium mitochondrial rps7 transcript processing

The wheat mitochondrial gene for ribosomal protein S7 exhibits multiple transcripts that share the same 3' terminus but range in overall length from 3.4 to 0.7 kb because of 5'end-maturation events. The longest detectable precursor RNA maps precisely to the 3' end of a chloroplast-origin tRNA-Phe gene, consistent with it providing signals for endonucleolytic cleavage. Steady-state levels of precursor RNAs were seen to be lower in seedlings than in germinating embryos, although the degree of editing within untranslated regions (UTRs) was higher in seedlings. In another grass, Lolium multiflorum Lam., rps7 displays transcripts of 1.3 and 0.7 kb, and although the distal 5' UTRs are unrelated in sequence to those of wheat, the 5' terminus of the longer transcript also maps to a tRNA gene, in this case the native mitochondrial-type tRNA-Ser. Our findings illustrate the plasticity of plant mitochondrial transcriptional units and the recruitment of chloroplast-origin sequences for the expression of mitochondrial genes.