Related Experiment Video
Updated: May 19, 2026
03:19
Point-of-Care Kidney and Genitourinary Ultrasound in Adults: Image Acquisition
Published on: June 21, 2024
Urinary proteome analysis in congenital bilateral hydronephrosis.
Lene Stødkilde1, Mia Gebauer Madsen, Johan Palmfeldt
1The Water and Salt Research Center, Institute of Clinical Medicine, University of Aarhus, Aarhus, Denmark.
Scandinavian Journal of Urology
|August 16, 2012
Summary
This study identified 33 urinary proteins related to congenital bilateral hydronephrosis. Five proteins were pinpointed as potential biomarkers for this congenital kidney disorder.
Area of Science:
- Biochemistry
- Nephrology
- Proteomics
Background:
- Congenital bilateral hydronephrosis involves abnormal kidney drainage in infants.
- Understanding pathophysiological processes is crucial for identifying biomarkers.
Purpose of the Study:
- To map urinary protein changes after relieving congenital bilateral hydronephrosis.
- To identify potential urinary biomarkers for congenital obstructive nephropathy.
Main Methods:
- Proteomics techniques and mass spectrometry were used to analyze urinary samples.
- Enzyme-linked immunosorbent assay (ELISA) validated changes in selected proteins.
Main Results:
- 33 proteins showed significantly altered urinary excretion post-obstruction relief.
- Fibrinogen, plasminogen, transthyretin, and transferrin levels decreased, while Tamm-Horsfall protein increased.
Conclusions:
- A proteomics strategy successfully identified proteins associated with congenital bilateral hydronephrosis.
- A panel of five proteins shows promise as urinary biomarkers for this condition.
Related Concept Videos
01:11Anatomy of the Genitourinary System I: Kidneys and Ureters
The upper urinary system comprises two kidneys and two ureters, which are crucial in filtering blood and forming urine.KidneysLocation and Structure:The kidneys are two bean-shaped organs positioned behind the peritoneum on either side of the spine.Kidneys are between the 12th thoracic (T12) and the 3rd lumbar (L3) vertebrae.The position of the liver causes the right kidney to sit slightly lower than the left.Protective Layers:Each kidney is enveloped in a tough, fibrous membrane called the...
01:20Physiology of the Genitourinary System III: Urine Concentration and Dilution
The kidneys concentrate or dilute urine to maintain water and electrolyte balance. Nephrons, particularly the loop of Henle, play a crucial role in this process through the countercurrent multiplication system. This system establishes a high osmolarity in the renal medulla, which is essential for water reabsorption. In the loop of Henle’s descending limb, water is reabsorbed into the surrounding medulla due to its permeability to water. In contrast, the ascending limb actively transports...
01:28Microbiota of the Urogenital Tract
The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
01:24Imaging Studies II: Ultrasonography
IntroductionUltrasonography, or renal ultrasound, is a noninvasive medical imaging technique that uses high-frequency sound waves to visualize the kidneys, ureters, bladder, and surrounding tissues.Indications for Urinary System UltrasonographyUrinary system ultrasonography is indicated in various clinical scenarios, such as:Kidney Stones (Urolithiasis): To detect and monitor the size and presence of kidney or urinary tract stones.Hydronephrosis: To assess the dilation of the renal pelvis and...
01:28Imaging Studies I: Kidney, Ureter, and Bladder Studies
Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
01:26Nephrotic Syndrome II : Assessment and Medical Management
IntroductionNephrotic syndrome is a kidney disorder marked by excessive protein loss in the urine, leading to various systemic complications. This condition often results from damage to the glomeruli—the kidney's filtering units—causing proteinuria, low blood protein levels, and fluid retention. Understanding the assessment, diagnosis, and management of nephrotic syndrome is essential for effective treatment and prevention of further kidney damage.AssessmentPatient History: Document any history...