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Updated: May 19, 2026
An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue
Published on: June 2, 2023
Microbeam-irradiated tumour tissue possesses a different infrared absorbance profile compared to broad beam and
Monica Sharma1, Jeffrey C Crosbie, Ljiljana Puskar
1University of Melbourne, Department of Obstetrics & Gynaecology, Royal Women's Hospital, Parkville, Victoria, Australia.
Purpose:
To investigate biochemical changes in mouse tumour tissue following Microbeam Radiation Therapy (MRT) and Broad Beam (BB) irradiation using synchrotron Fourier-Transform Infrared (FTIR) microspectroscopy.
Materials And Methods:
Synchrotron FTIR microspectroscopy was carried out on mouse tumour sections previously irradiated with BB (11, 22 or 44 Gy), MRT (560 Gy in-beam, 25 μm wide, 200 μm peak separation) or sham-irradiation (0 Gy) from mice culled 4 hours post-irradiation.
Results:
MRT and BB-irradiated tumour sections showed clear chemical shifts in spectral bands corresponding to functional group vibrations in protein (1654-1630 cm(-1)), lipid (~1470, 1463 cm(-1)) and nucleic acid (1130-1050 cm(-1)). MRT peak and valley regions showed virtually identical absorbance patterns in protein and lipid regions. However, we observed chemical shifts corresponding to the nucleic acid region (1120-1050 cm(-1)) between the peak and valley dose regions. Chemical maps produced from integrating absorbance bands of interest over the scanned tumour area did not reveal any microbeam paths.
Conclusions:
The lack of difference between MRT peak and valley irradiated areas suggests a holistic tissue response to MRT that occurs within 4 h, and might be the first evidence for a mechanism by which MRT kills the whole tumour despite only a small percentage receiving peak irradiation.
