The potential of a niacinamide dominated cosmeceutical formulation on fibroblast activity and wound healing in vitro
Related Experiment Video
Updated: May 19, 2026
Formulation of Zinc-Based Nanomaterials using the Eucommia ulmoides Bark Extract and their Wound Healing Potential
Published on: December 27, 2024
Quenton Wessels1, Etheresia Pretorius, Celeste M Smith
1Department of Anatomy, School of Medicine, University of Namibia, Windhoek, NamibiaDepartment of Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, South AfricaSouthern group of Companies, Southern Implants Office Park, Irene, South Africa.
Abstract:
Knowledge on the intrinsic mechanisms involved in wound healing provides opportunity for various therapeutic strategies. The manipulation of dermal fibroblast proliferation and differentiation might prove to beneficially augment wound healing. This study evaluated the combined effects of niacinamide, L-carnosine, hesperidin and Biofactor HSP(®) on fibroblast activity. The effects on fibroblast collagen production, cellular proliferation, migration and terminal differentiation were assessed. In addition, the authors determined the effects on in vitro wound healing. The optimal concentrations of actives were determined in vitro. Testing parameters included microscopic morphological cell analysis, cell viability and proliferation determination, calorimetric collagen detection and in vitro wound healing dynamics. Results show that 0·31 mg/ml niacinamide, 0·10 mg/ml L-carnosine, 0·05 mg/ml hesperidin and 5·18 µg/ml Biofactor HSP® proved optimal in vitro. The results show that fibroblast collagen synthesis was increased alongside with cellular migration and proliferation.
