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Updated: May 19, 2026

Longitudinal Micro-Computed Tomography Image Analysis for User-Defined Region of Interest in Critical-Sized Bone Defects
Published on: June 24, 2025
The long leg radiograph is a reliable method of assessing alignment when compared to computer-assisted navigation and
Sina Babazadeh1, Michelle M Dowsey, Roger J Bingham
1Department of Orthopaedics, St. Vincent's Hospital, and Department of Surgery, The University of Melbourne, Australia.
Background:
The mechanical alignment of the knee is an important factor in planning for, and subsequently assessing the success of a knee replacement. It is most commonly measured using a long-leg anteroposterior radiograph (LLR) encompassing the hip, knee and ankle. Other modalities of measuring alignment include computer tomography (CT) and intra-operative computer navigation (Cas). Recent studies comparing LLRs to Cas in measuring alignment have shown significant differences between the two and have hypothesized that Cas is a more accurate modality. This study aims to investigate the accuracy of the above mentioned modalities.
Methodology:
A prospective study was undertaken comparing alignment as measured by long-leg radiographs and computer tomography to intra-operative navigation measurements in 40 patients undergoing a primary total knee replacement to test this hypothesis. Alignment was measured three times by three observers. Intra- and inter-observer correlation was sought between modalities.
Results:
Intra-observer correlation was excellent in all cases (>0.98) with a coefficient of repeatability <1.1°. Inter-observer correlation was also excellent measuring >0.960 using LLRs and >0.970 using CT with coefficient of repeatability <2.8°. Inter-modality correlation proved to be higher when comparing LLRs and CT (>0.893), than when comparing either of these modalities with Cas (>0.643 and >0.671 respectively). Pre-operative values had the greatest variability.
Conclusion:
Given its availability and reduced radiation dose when compared to CT, LLRs should remain the mainstay of measuring the mechanical alignment of the lower limb, especially post-operatively.
Level Of Evidence:
II.
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