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Updated: May 19, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Role of smart polymers in protein purification and refolding
Saurabh Gautam1, Priyanka Dubey, Raghavan Varadarajan
1Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India.
Abstract:
Affinity precipitation is a non-chromatographic method which is useful for purification and refolding of proteins. Quite often, a stimuli-sensitive polymer can be identified which selectively binds to the desired protein. For separation, the protein can be recovered from the precipitate of the protein-smart polymer complex. In case of a refolding experiment, binding of the solubilized protein (in its denatured form) with the polymer leads to the refolding of the protein.
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