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Updated: May 19, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
Published on: May 29, 2012
Deep ultraviolet resonant Raman imaging of a cell
Yasuaki Kumamoto1, Atsushi Taguchi, Nicholas Isaac Smith
1RIKEN, Nanophotonics Laboratory, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Abstract:
We report the first demonstration of deep ultraviolet (DUV) Raman imaging of a cell. Nucleotide distributions in a HeLa cell were observed without any labeling at 257 nm excitation with resonant bands attributable to guanine and adenine. Obtained images represent DNA localization at nucleoli in the nucleus and RNA distribution in the cytoplasm. The presented technique extends the potential of Raman microscopy as a tool to selectively probe nucleic acids in a cell with high sensitivity due to resonance.
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